How to bust up a bacterial biofilm
How to bust up a bacterial biofilm
Air date: Wednesday, March 27, 2019, 3:00:00 PM
Category: WALS - Wednesday Afternoon Lectures
Runtime: 00:50:39
Description: NIH Director's Wednesday Afternoon Lecture Series
The Bakaletz laboratory’s research focus is attempting to understand the pathogenic mechanisms operational in the highly prevalent pediatric disease, otitis media (OM) (or middle ear infection). Specifically, we are interested in elucidating how upper respiratory tract viruses predispose the middle ear to invasion by any of the three predominant bacterial pathogens of OM (nontypeable Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pneumoniae). We are also interested in understanding how bacterial biofilms contribute to the recurrence and chronicity of OM, chronic rhinosinusitis, bronchitis, chronic cough and exacerbations of both COPD and CF. With this enhanced understanding, we then attempt to identify potential molecular targets for the design, development and pre-clinical testing of novel vaccine candidates or therapeutics using an animal model of viral-bacterial superinfection that closely mimics the polymicrobial nature of OM in children. Our long-term goal is to develop novel methods to more effectively treat or preferably, to prevent, otitis media and other respiratory tract infections where there is a biofilm component to the disease course.
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Lauren O. Bakaletz, Ph.D., Professor of Pediatrics and Otolaryngology, Vice President for Basic Sciences, Director, Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital
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Born Racer
A powerful and inspirational story of dedication, danger, fear, and the rare 'will' some of us have to defy all personal limitations. Experience one of the fastest motorsports on earth through the eyes of four-time world champion Scott Dixon and the Chip Ganassi Racing team. Filmed with an access all areas lens, 'Born Racer' follows the people who are passionate about the world of auto racing and asks why some individuals feel compelled to face danger and risk their lives in order to win. Both action-packed and highly-intimate it features an intense blend of up close and personal filming with never-before-seen spectacular, cutting-edge racing footage to explore a sport that defines the very people who inhabit it, and pushes them to the edge in their desire for success.
How Does a Transistor Work?
How does a transistor work? Our lives depend on this device.
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When I mentioned to people that I was doing a video on transistors, they would say as in a transistor radio? Yes! That's exactly what I mean, but it goes so much deeper than that. After the transistor was invented in 1947 one of the first available consumer technologies it was applied to was radios, so they could be made portable and higher quality. Hence the line in 'Brown-eyed Girl' - going down to the old mine with a transistor radio.
But more important to our lives today, the transistor made possible the microcomputer revolution, and hence the Internet, and also TVs, mobile phones, fancy washing machines, dishwashers, calculators, satellites, projectors etc. etc. A transistor is based on semiconductor material, usually silicon, which is 'doped' with impurities to carefully change its electrical properties. These n and p-type semiconductors are then put together in different configurations to achieve a desired electrical result. And in the case of the transistor, this is to make a tiny electrical switch. These switches are then connected together to perform computations, store information, and basically make everything electrical work intelligently.
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And thanks to Henry Reich and Vanessa Hill for reviews of earlier drafts of this video.
Music: Kevin MacLeod (incompetech.com) Decisions
Scorpions - Under The Same Sun (Official Music Video)
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Music video by Scorpions performing Under The Same Sun. (C) 1993 The Island Def Jam Music Group
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Bob Ross - A Walk in the Woods (Season 1 Episode 1)
Bob Ross introduces us to his Almighty assortment of tools and colors, tells us that anyone can paint, and creates a landscape of a forest path just after a rain shower.
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Originally aired on 1/11/1983
Biblical Series I: Introduction to the Idea of God
Lecture I in my Psychological Significance of the Biblical Stories series from May 16th at Isabel Bader Theatre in Toronto. In this lecture, I describe what I consider to be the idea of God, which is at least partly the notion of sovereignty and power, divorced from any concrete sovereign or particular, individual person of power. I also suggest that God, as Father, is something akin to the spirit or pattern inherent in the human hierarchy of authority, which is based in turn on the dominance hierarchies characterizing animals.
Q & A Starts: 1:57:25
Producer Credit and thanks to the following $200/month Patreon supporters. Without such support, this series would not have happened: Adam Clarke, Alexander Meckhai’el Beraeros, Andy Baker, Arden C. Armstrong, Badr Amari, BC, Ben Baker, Benjamin Cracknell, Brandon Yates, Chad Grills, Chris Martakis, Christopher Ballew, Craig Morrison, Daljeet Singh, Damian Fink, Dan Gaylinn, Daren Connel, David Johnson, David Tien, Donald Mitchell, Eleftheria Libertatem, Enrico Lejaru, George Diaz, GeorgeB, Holly Lindquist, Ian Trick, James Bradley, James N. Daniel, III, Jan Schanek, Jason R. Ferenc, Jesse Michalak, Joe Cairns, Joel Kurth, John Woolley, Johnny Vinje, Julie Byrne, Keith Jones, Kevin Fallon, Kevin Patrick McSurdy, Kevin Van Eekeren, Kristina Ripka, Louise Parberry, Matt Karamazov, Matt Sattler, Mayor Berkowitz , Michael Thiele, Nathan Claus, Nick Swenson , Patricia Newman, Robb Kelley, Robin Otto, Ryan Kane, Sabish Balan, Salman Alsabah, Scott Carter, Sean C., Sean Magin, Sebastian Thaci, Shiqi Hu, Soheil Daftarian, Srdan Pavlovic, Starting Ideas, Too Analytical, Trey McLemore, William Wilkinson, Yazz Troche, Zachary Vader
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FLO Cycling - Installing the Fork, Stem, & Handlebar
This video is part of the FLO Cycling How to Build a Road Bike e-Book/Video Series. You can download the accompanying e-Book at to learn how to build a road bike from scratch.
LPG Safety Film
Watch this film to know some important points on how to keep safety in the kitchen. How to make sure if the stove, gas pipe & cylinder are in good condition.
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The Backwards Brain Bicycle - Smarter Every Day 133
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Here's the link from the Amsterdam meetup!
(I usually make a localized facebook post before I visit a city to see if anyone wants to high five.)
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Slow Motion Sound Design by A Shell In The Pit
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Bottles which can be downloaded here.
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A special thanks to these guys for helping me make the bikes in Australia.
Brian - @weezmgk
Tom - @SydneyTom_
Bib Bishop - Actually turned the gears down in his shop. Couldn't have done it without Bob!
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A quick clarification....
It took me 8 months to learn how to do this, but I was only picking up the bike and running to the end of the driveway and back every day. I wasn't ACTIVELY trying to learn. Meaning... I wasn't struggling and trying to make my brain learn. I simply got on the bike every day, tried to operate it to the end of the driveway, turned around and tried to operate it back. The goal was to understand how my brain figured things out on its own, without trying to force it to. Many people have built bikes like this and figured it out in much less than 1 day by staying on the bike until they were able to master it. I had no timelines, and was using this as an exploratory activity to learn how I learn.
Do not misinterpret this to mean that I struggled and tried very hard every day for 8 months. That's simply not true.
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Waste and Webs | Critical Role | Campaign 2, Episode 10
The Mighty Nein delve into the sewers of Zadash in search of the mysterious beast that has been terrorizing the Crownsguard...
Thanks to Far Cry 5 and D&D Beyond for sponsoring this episode of Critical Role!
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Far Cry 5 comes out on March 27th for PS4, Xbox One, and PC – go to to learn more and pick up a copy. Also be sure to check out this video featuring Nerdist’s Dan Casey, Kyle Hill, and our very own Erika Ishii as they face challenges IRL that players will take on in Far Cry 5 -- tell them Critical Role sent you!
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The Howling Mines | Critical Role | Campaign 2, Episode 6
The group questions the crownsguard for clarity about last night’s attacks, and their hunt for the gnolls leads them to a descent down a mysterious mine...
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Inclusive Voices: Welcoming People with Disabilities
October 7th, 2019 Hartford Seminary and the UConn Center for Excellence in Developmental Disabilities held this interfaith conference on creating communities of belonging.
PSXplosion #193 Monster Rancher 2 [Part 4]. Later: King's Quest: Mask of Eternity [Part 4]
Chat is being run through my Discord server! The stream and chat are both available from
Backseat/hints = ban.
The Puri stream -- this channel streams playthroughs of a variety of games, genres, and platforms. Chat is run through my Discord server. An invite link can be had through my site at
Wind River Official Trailer #1 (2017) Jeremy Renner, Elizabeth Olsen Thriller Movie HD
Wind River Trailer 1 (2017) Jeremy Renner, Elizabeth Olsen Thriller Movie HD [Official Trailer]
Iran, SAVAK, and the CIA: Financial Support and Training
SAVAK (Persian: ساواک, short for سازِمانِ اطلاعات وَ امنیَتِ کِشوَر Sāzemān-e Ettelā'āt va Amniyat-e Keshvar, Organisation of Intelligence and National Security) was the secret police, domestic security and intelligence service established by Iran's Mohammad Reza Shah with the help of the United States' Central Intelligence Agency (the CIA). SAVAK operated from 1957 to 1979, when the Pahlavi dynasty was overthrown. SAVAK has been described as Iran's most hated and feared institution prior to the revolution of 1979 because of its practice of torturing and executing opponents of the Pahlavi regime. At its peak, the organization had as many as 60,000 agents serving in its ranks according to one source, although Gholam Reza Afkhami, whose work on the Shah has been described as a sympathetic biography, estimates SAVAK staffing at between 4,000 and 6,000.
After removing the populist regime of Mohammad Mosaddeq (which was originally focused on nationalizing Iran's oil industry but also set out to weaken the Shah's power) from power on 19 August 1953, in a coup, the monarch, Mohammad Reza Shah, established an intelligence service with police powers. The Shah's goal was to strengthen his regime by placing political opponents under surveillance and repress dissident movements. According to Encyclopædia Iranica:
A U.S. Army colonel working for the CIA was sent to Persia in September 1953 to work with General Teymur Bakhtiar, who was appointed military governor of Tehran in December 1953 and immediately began to assemble the nucleus of a new intelligence organization. The U.S. Army colonel worked closely with Bakhtīār and his subordinates, commanding the new intelligence organization and training its members in basic intelligence techniques, such as surveillance and interrogation methods, the use of intelligence networks, and organizational security. This organization was the first modern, effective intelligence service to operate in Persia. Its main achievement occurred in September 1954, when it discovered and destroyed a large communist Tudeh Party network that had been established in the Persian armed forces.
In March 1955, the Army colonel was replaced with a more permanent team of five career CIA officers, including specialists in covert operations, intelligence analysis, and counterintelligence, including Major General Herbert Norman Schwarzkopf who trained virtually all of the first generation of SAVAK personnel. In 1956 this agency was reorganized and given the name Sazeman-e Ettela'at va Amniyat-e Keshvar (SAVAK). These in turn were replaced by SAVAK's own instructors in 1965. Chief CIA Iran analyst Jesse Leaf in an interview on 6th Jan. 1979 stated that the CIA taught Nazi torture techniques to SAVAK.
SAVAK had the power to censor the media, screen applicants for government jobs, and according to reliable Western source, use all means necessary, including torture, to hunt down dissidents. The CIA provided SAVAK with lists of Communists to torture and murder. These lists originated with KGB defectors working for the CIA.
After 1963, the Shah expanded his security organizations, including SAVAK, which grew to over 5300 full-time agents and a large but unknown number of part-time informers.
In 1961 the Iranian authorities dismissed the agency's first director, General Teymur Bakhtiar; he later became a political dissident. In 1970 SAVAK agents assassinated him, disguising the deed as an accident.
Sources disagree over how many victims SAVAK had and how inhumane its techniques were. Writing at the time of the Shah's overthrow, TIME magazine described SAVAK as having long been Iran's most hated and feared institution which had tortured and murdered thousands of the Shah's opponents. The Federation of American Scientists also found it guilty of the torture and execution of thousands of political prisoners and symbolizing the Shah's rule from 1963-79. The FAS list of SAVAK torture methods included electric shock, whipping, beating, inserting broken glass and pouring boiling water into the rectum, tying weights to the testicles, and the extraction of teeth and nails. According to a former CIA analyst on Iran, Jesse J. Leaf, SAVAK was trained in torture techniques by the CIA.
Puri Plays: Shenmue III [Part 1] (PC, 2019)
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CFSAC 2014 Spring Meeting: ME/CFS Researcher and Clinician
The Chronic Fatigue Syndrome Advisory Committee (CFSAC) meets on day one of their 2014 Spring Meeting.
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nvfc 3 Live https://goo.gl/SwT1Z3 NOVAFAMIILYCHURCH LIVE
THIS IS THE NEWEST LIVE STREAM FOR NOVAFAMILY CHURCH
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The Hobbit: An Unexpected Journey
Academy Award®-winning filmmaker Peter Jackson returns to Middle Earth with the first of three films based on J.R.R. Tolkien's enduring masterpiece. Set in Middle Earth 60 years before the epic Lord of the Rings trilogy,the adventure follows the journey of Bilbo Baggins,who is swept into an epic quest to reclaim the lost Dwarf Kingdom from the fearsome dragon Smaug. Approached out of the blue by the wizard Gandalf,Bilbo finds himself joining a company of dwarves on a journey into wild,treacherous lands swarming with beasts of every ilk. Although their goal lies to the East,they must first escape the goblin tunnels,where Bilbo meets the creature that will change his life forever...Gollum. Alone with Gollum,on the shores of an underground lake,the unassuming Bilbo gains possession of Gollum's precious -- a simple,gold ring tied to the fate of all Middle Earth.
Как накачать (нарастить) огромный бицепс и мышцы рук?
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